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pcdna3 1 expression vector  (TaKaRa)


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    TaKaRa pcdna3 1 expression vector
    Pcdna3 1 Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 7418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+expression+vector/T4+DNA+Ligase/pm41148321-70-7-15
    Average 97 stars, based on 7418 article reviews
    pcdna3 1 expression vector - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Transfection:

    Article Title: Human Daxx-mediated Repression of Human Cytomegalovirus Gene Expression Correlates with a Repressive Chromatin Structure around the Major Immediate Early Promoter
    Article Snippet: .. Plasmids and Transfections—pCDNA3hDaxx contains a full-length cDNA of hDaxx in the pCDNA3 expression vector. pDsRed (Clontech) encodes a novel RFP under the control of the minimal HCMVMIEP. pDsRed-hDaxx (a kind gift of Richard Caswell, Cardiff University) consists of the full-length hDaxx cDNA, minus the stop codon, cloned into EcoRI/ BamHI-digested sites in pDsRed1-N1. ..

    Expressing:

    Article Title: Human Daxx-mediated Repression of Human Cytomegalovirus Gene Expression Correlates with a Repressive Chromatin Structure around the Major Immediate Early Promoter
    Article Snippet: .. Plasmids and Transfections—pCDNA3hDaxx contains a full-length cDNA of hDaxx in the pCDNA3 expression vector. pDsRed (Clontech) encodes a novel RFP under the control of the minimal HCMVMIEP. pDsRed-hDaxx (a kind gift of Richard Caswell, Cardiff University) consists of the full-length hDaxx cDNA, minus the stop codon, cloned into EcoRI/ BamHI-digested sites in pDsRed1-N1. ..

    Article Title: Regulation of TRPC6 Channel Activity by Tyrosine Phosphorylation
    Article Snippet: .. The fragment was inserted into the EcoRI-BamHI sites of pcDNA3 expression vector (Clontech). ..

    Article Title: NMR Solution Structure of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase in Complex with a Paxillin LD Peptide
    Article Snippet: .. For expression in 293 cells, wild type FAK and mutants were subcloned into the pcDNA3 expression vector (Clontech). .. Cells were transfected using Lipofectamine according to the manufacturer’s recommendations (Invitrogen).

    Article Title: Increased p190RhoGEF expression in activated B cells correlates with the induction of the plasma cell differentiation
    Article Snippet: .. To generate expression plasmids for WT and DN p190RhoGEF (MIGR1), the cDNAs encoding WT and DN p190RhoGEF were released from the pcDNA3 expression vector by digestion with Bgl II and Not I and ligated into the pLNCX2 retroviral vector (Clontech Laboratories, Inc., Palo Alto, CA) before being sub-cloned into the MSCV MIGR1 bicistronic vector (a kind gift from W. Pear through G. Koretzky, University of Pennsylvania) , between the Bgl II and Sal I/ Xho I sites. .. Plasmids expressing FLAG-tagged CA (Q63L) and DN (T19N) RhoA (MIGR1) were generated by PCR amplification with FLAG sequence-containing primers from CA and DN RhoA (pRK5-myc) expression plasmids (kind gifts from Dr. G. M. Bokoch, The Scripps Research Institute, La Jolla, CA), followed by ligation into the MSCV MIGR1 bicistronic vector between the Bgl II and Eco RI sites.

    Control:

    Article Title: Human Daxx-mediated Repression of Human Cytomegalovirus Gene Expression Correlates with a Repressive Chromatin Structure around the Major Immediate Early Promoter
    Article Snippet: .. Plasmids and Transfections—pCDNA3hDaxx contains a full-length cDNA of hDaxx in the pCDNA3 expression vector. pDsRed (Clontech) encodes a novel RFP under the control of the minimal HCMVMIEP. pDsRed-hDaxx (a kind gift of Richard Caswell, Cardiff University) consists of the full-length hDaxx cDNA, minus the stop codon, cloned into EcoRI/ BamHI-digested sites in pDsRed1-N1. ..

    Clone Assay:

    Article Title: Human Daxx-mediated Repression of Human Cytomegalovirus Gene Expression Correlates with a Repressive Chromatin Structure around the Major Immediate Early Promoter
    Article Snippet: .. Plasmids and Transfections—pCDNA3hDaxx contains a full-length cDNA of hDaxx in the pCDNA3 expression vector. pDsRed (Clontech) encodes a novel RFP under the control of the minimal HCMVMIEP. pDsRed-hDaxx (a kind gift of Richard Caswell, Cardiff University) consists of the full-length hDaxx cDNA, minus the stop codon, cloned into EcoRI/ BamHI-digested sites in pDsRed1-N1. ..

    Plasmid Preparation:

    Article Title: Regulation of TRPC6 Channel Activity by Tyrosine Phosphorylation
    Article Snippet: .. The fragment was inserted into the EcoRI-BamHI sites of pcDNA3 expression vector (Clontech). ..

    Article Title: NMR Solution Structure of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase in Complex with a Paxillin LD Peptide
    Article Snippet: .. For expression in 293 cells, wild type FAK and mutants were subcloned into the pcDNA3 expression vector (Clontech). .. Cells were transfected using Lipofectamine according to the manufacturer’s recommendations (Invitrogen).

    Retroviral:

    Article Title: Increased p190RhoGEF expression in activated B cells correlates with the induction of the plasma cell differentiation
    Article Snippet: .. To generate expression plasmids for WT and DN p190RhoGEF (MIGR1), the cDNAs encoding WT and DN p190RhoGEF were released from the pcDNA3 expression vector by digestion with Bgl II and Not I and ligated into the pLNCX2 retroviral vector (Clontech Laboratories, Inc., Palo Alto, CA) before being sub-cloned into the MSCV MIGR1 bicistronic vector (a kind gift from W. Pear through G. Koretzky, University of Pennsylvania) , between the Bgl II and Sal I/ Xho I sites. .. Plasmids expressing FLAG-tagged CA (Q63L) and DN (T19N) RhoA (MIGR1) were generated by PCR amplification with FLAG sequence-containing primers from CA and DN RhoA (pRK5-myc) expression plasmids (kind gifts from Dr. G. M. Bokoch, The Scripps Research Institute, La Jolla, CA), followed by ligation into the MSCV MIGR1 bicistronic vector between the Bgl II and Eco RI sites.



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    ( A – E ) Distribution of ATP5H ( A ), NDUFC1 ( B ), <t>NDUFV2</t> ( C ), SLC25A4 ( D ), and UCP2 ( E ) mRNAs across ribosome fractions (free RNA/RNP, 40S/60S/80S, light polysome, heavy polysome) from sgNC and sgMETTL1 BxPC-3 and MiaPaCa-2 cells, quantified by qRT-PCR. Bar graphs show the proportion of untranslated and translated fractions. ** P < 0.01 and *** P < 0.001, by 2-tailed, unpaired t test. ( F ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and METTL1-KO cells by Western blotting. ( G ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and valine tRNA–KD cells by Western blotting. ( H and I ) Oxygen consumption rate in control and METTL1-KO groups from BxPC-3 ( H ) and MiaPaCa-2 ( I ) cells by Seahorse Mito Stress testing. *** P < 0.001, by 1-way ANOVA followed by Dunnett’s multiple-comparison test. ( J and K ) Oxygen consumption rate in control and valine tRNA–KD group from BxPC-3 ( J ) and MiaPaCa-2 ( K ) cells by Seahorse Mito Stress testing. *** P < 0.001, by1-way ANOVA followed by Dunnett’s multiple-comparison test. Data are representative of 3 independent experiments. All data are presented as the mean ± SD.
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    ( A – E ) Distribution of ATP5H ( A ), NDUFC1 ( B ), <t>NDUFV2</t> ( C ), SLC25A4 ( D ), and UCP2 ( E ) mRNAs across ribosome fractions (free RNA/RNP, 40S/60S/80S, light polysome, heavy polysome) from sgNC and sgMETTL1 BxPC-3 and MiaPaCa-2 cells, quantified by qRT-PCR. Bar graphs show the proportion of untranslated and translated fractions. ** P < 0.01 and *** P < 0.001, by 2-tailed, unpaired t test. ( F ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and METTL1-KO cells by Western blotting. ( G ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and valine tRNA–KD cells by Western blotting. ( H and I ) Oxygen consumption rate in control and METTL1-KO groups from BxPC-3 ( H ) and MiaPaCa-2 ( I ) cells by Seahorse Mito Stress testing. *** P < 0.001, by 1-way ANOVA followed by Dunnett’s multiple-comparison test. ( J and K ) Oxygen consumption rate in control and valine tRNA–KD group from BxPC-3 ( J ) and MiaPaCa-2 ( K ) cells by Seahorse Mito Stress testing. *** P < 0.001, by1-way ANOVA followed by Dunnett’s multiple-comparison test. Data are representative of 3 independent experiments. All data are presented as the mean ± SD.
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    Image Search Results


    ( A – E ) Distribution of ATP5H ( A ), NDUFC1 ( B ), NDUFV2 ( C ), SLC25A4 ( D ), and UCP2 ( E ) mRNAs across ribosome fractions (free RNA/RNP, 40S/60S/80S, light polysome, heavy polysome) from sgNC and sgMETTL1 BxPC-3 and MiaPaCa-2 cells, quantified by qRT-PCR. Bar graphs show the proportion of untranslated and translated fractions. ** P < 0.01 and *** P < 0.001, by 2-tailed, unpaired t test. ( F ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and METTL1-KO cells by Western blotting. ( G ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and valine tRNA–KD cells by Western blotting. ( H and I ) Oxygen consumption rate in control and METTL1-KO groups from BxPC-3 ( H ) and MiaPaCa-2 ( I ) cells by Seahorse Mito Stress testing. *** P < 0.001, by 1-way ANOVA followed by Dunnett’s multiple-comparison test. ( J and K ) Oxygen consumption rate in control and valine tRNA–KD group from BxPC-3 ( J ) and MiaPaCa-2 ( K ) cells by Seahorse Mito Stress testing. *** P < 0.001, by1-way ANOVA followed by Dunnett’s multiple-comparison test. Data are representative of 3 independent experiments. All data are presented as the mean ± SD.

    Journal: The Journal of Clinical Investigation

    Article Title: METTL1-mediated m 7 G modification of valine tRNAs drives metabolic adaptation in pancreatic ductal adenocarcinoma cells

    doi: 10.1172/JCI204312

    Figure Lengend Snippet: ( A – E ) Distribution of ATP5H ( A ), NDUFC1 ( B ), NDUFV2 ( C ), SLC25A4 ( D ), and UCP2 ( E ) mRNAs across ribosome fractions (free RNA/RNP, 40S/60S/80S, light polysome, heavy polysome) from sgNC and sgMETTL1 BxPC-3 and MiaPaCa-2 cells, quantified by qRT-PCR. Bar graphs show the proportion of untranslated and translated fractions. ** P < 0.01 and *** P < 0.001, by 2-tailed, unpaired t test. ( F ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and METTL1-KO cells by Western blotting. ( G ) Relative protein levels of NDUFV2 and UCP2 in PDAC control and valine tRNA–KD cells by Western blotting. ( H and I ) Oxygen consumption rate in control and METTL1-KO groups from BxPC-3 ( H ) and MiaPaCa-2 ( I ) cells by Seahorse Mito Stress testing. *** P < 0.001, by 1-way ANOVA followed by Dunnett’s multiple-comparison test. ( J and K ) Oxygen consumption rate in control and valine tRNA–KD group from BxPC-3 ( J ) and MiaPaCa-2 ( K ) cells by Seahorse Mito Stress testing. *** P < 0.001, by1-way ANOVA followed by Dunnett’s multiple-comparison test. Data are representative of 3 independent experiments. All data are presented as the mean ± SD.

    Article Snippet: The NDUFV2 expression vector (pcDNA3.1/NDUFV2/myc-His) was obtained from Addgene (127491).

    Techniques: Quantitative RT-PCR, Control, Western Blot, Comparison